Eva Nogales, Professor

Closed (1) Project 1: Structural studies of yeast septins and interacting partners

Closed. This professor is continuing with Spring 2017 apprentices on this project; no new apprentices needed for Fall 2017.

Septins are a group of proteins found in a majority of eukaryotic cells that may assemble into filaments, rings, and gauzes. These complex structures of septins then aid in cell division by acting as scaffold to recruit other proteins and by creating a diffusion barrier to prevent other factors from moving between cells.

We are looking for someone to aid in expression and purification of septins and septin binding-proteins and to learn how to use electron microscopy in order to study the interaction that occurs between them. There will be opportunities to learn some cloning techniques, fabrication of lipid monolayers and bilayers, and an introduction to cryo-electron microscopy.

We are looking for someone to aid in expression and purification of septins and septin binding-proteins and to learn how to use electron microscopy in order to study the interaction that occurs between them. There will be opportunities to learn some cloning techniques, fabrication of lipid monolayers and bilayers, and an introduction to cryo-electron microscopy.

Day-to-day supervisor for this project: Sarah Sterling, Post-Doc

Qualifications: The candidate should have some basic knowledge of biochemistry and or basic physics. He or she should be able to keep good record of his/her progress and to work in a team.

Weekly Hours: 9-12 hrs

Related website: http://cryoem.berkeley.edu

Closed (2) Computational model of atomic structrues into high resolution cryo-EM maps of microtubules

Closed. This professor is continuing with Spring 2017 apprentices on this project; no new apprentices needed for Fall 2017.

We are interested in the ultrastructure of chlorplasts in order to understand processes light light-adjustment or chloroplast biogenesis. Towards our goal we use electron microscopy imaging and computational image analysis.

The candidate will be involved in the study of chloroplasts in Chlamydomonas by tomography. She/He will perform specimen preparation (embedding, ultramicrotomy) both for TEM and FIB/SEM as well as 3D reconstruction and data analysis.

Day-to-day supervisor for this project: Liz Kellogg, Post-Doc

Qualifications: The candidate should have some basic knowledge of biochemistry and or basic physics. He or she should be able to keep good record of his/her progress and to work in a team.

Weekly Hours: 9-12 hrs

Closed (3) Structure-function studies of large macromolecular complexes using biochemistry and cryo-electron microscopy

Applications for Fall 2017 are now closed for this project.

A mechanistic understanding of biological functions needs a structural description of the macromolecular assemblies that carry out those functions. We are using cryo-electron microscopy, a new and powerful structural biology technique, to describe the architecture, dynamics and interactions of complexes involved in gene expression, both at the transcription initiation level and during silencing via epigenetic marks.

The student will be involved in the expression and/or purification of protein components, the biochemical characterization, and the analysis via electron microscopy visualization

Day-to-day supervisor for this project: Robert Louder, Graduate Student

Qualifications: Knowledge of basic principles of biochemistry desirable. Strong work ethics, attention to detail, and team spirit are required. Previous experience in biochemistry or molecular biology technique is desirable.

Weekly Hours: 9-12 hrs

Related website: cryoem.berkeley.edu