Guo Huang, Professor

Open (1) Molecular control of organ regeneration in zebrafish and mice

Open. Apprentices needed for the fall semester. Please do NOT contact faculty before September 11th (the start of the 4th week of classes)! Enter your application on the web beginning August 16th. The deadline to apply is Tuesday, August 29th at 8 AM.

We aim to understand the divergent regenerative potential in ontogeny and phylogeny. For example, heart regeneration is remarkably robust in adult zebrafish and newborn mice while very limited in adult mammals. We use the heart as a model system and an entry point to study organ regeneration and repair in adult zebrafish, neonatal and adult mice, with an emphasis on the pathways that regulate resident stem cell activation and mature cell dedifferentiation/proliferation, and with innovative and integrated approaches in engineering, single cell analysis, advanced imaging microscopy and genome manipulation technology.

Specific questions include:

1. How does the heart regenerate in newborn mice and adult zebrafish?

2. What triggers the loss of cardiac regenerative potential in the mammalian postnatal life?

3. Can we reactivate regenerative potentials in adult mammalian hearts, and if so, how?

4. Are there species in higher vertebrates with extraordinary yet previously unknown tissue regenerative capacity?

5. Are there any human conditions or diseases that may result in preservation of cardiac regenerative potential? If so, how?

Our research will shed lights on the fundamental principle governing organ regeneration in development and evolution, and may suggest novel approaches of regenerative medicine.

Candidates will work with postdoctoral fellows and staff researchers in the lab. Students will also have the opportunity to engage directly with the professor.
Potential techniques and experience acquired during the training include:
- Advanced imaging microscopy on live or fixed tissues and animals;
- Molecular cloning and genome editing using the CRISPR technology;
- Mammalian and zebrafish cell culture;
- Mice and zebrafish husbandy, genotyping, and injury/regeneration models.

Day-to-day supervisor for this project: Alex Payumo, Post-Doc

Qualifications: This position is open to any sophomores, juniors and seniors who are highly motivated and very organized. Please include in the application (1) your career plan, (2) daily schedule of committed hours to perform research in the lab (please exclude commute time), (3) prior research experience if any, and (4) any prior experience of painting/photography or related experience that demonstrates strong visual artistic senses.

Weekly Hours: more than 12 hrs

Off-Campus Research Site: 555 Mission Bay Blvd S, San Francisco, CA 94158

Related website: http://www.cvri.ucsf.edu/~huang/lab/Research.html