The role of myo-inositol and the microbiome in hummingbird gut metabolism
Michael Shapira, Professor
Integrative Biology
Applications for Spring 2026 are closed for this project.
Nectar-feeding birds rely on extremely high metabolic rates and often accumulate fat as an adaptive strategy for migration, fasting, or periods of limited food availability. Recent work across animals suggests that gut microbes play an important role in regulating host metabolism and fat accumulation. In particular, the dietary compound myo-inositol (MI) has been shown in mammals to influence lipid absorption through interactions between the gut microbiome and intestinal cells. However, whether similar mechanisms operate in birds, especially nectarivorous species, remains unknown.
This project will investigate how dietary MI influences gut microbial composition, microbial metabolism, and gut efficiency in nectar-feeding birds. By combining microbiome analyses, cell culture approaches, and in vitro microbial growth experiments, the project aims to disentangle direct effects of MI on gut cells from indirect effects mediated by gut microbes.
Role: The undergraduate researcher will assist with several aspects of the project, which may include:
• Collecting and processing fecal samples from birds fed control or MI-supplemented nectar
• Assisting with microbiome sample preparation and data analysis
• Helping establish and maintain avian gut epithelial cell cultures
• Performing basic wet-lab assays related to metabolism
• Reading scientific literature and contributing to interpretation of results
The student will receive hands-on training in laboratory techniques, experimental design, and data analysis.
Time commitment and work format
• 9–11 hours per week
• Work is primarily in person in the lab
• Ability to work in 2–3 hour blocks is preferred
Qualifications: No prior research experience is required; all necessary training will be provided. Students with previous laboratory experience are encouraged to apply.
Beyond experience, we seek students who are curious and engaged.
Hours: 9-11 hrs
Biological & Health Sciences Engineering, Design & Technologies