How specialized cells detect changes in pressure for normal hearing and balance in zebrafish
Ian Swinburne, Professor
Molecular and Cell Biology
Closed. This professor is continuing with Spring 2024 apprentices on this project; no new apprentices needed for Fall 2024.
Most life processes involve cells managing fluids. Our sense of hearing and balance depend on the tight regulation of inner ear fluid (endolymph) volume and pressure. Increased endolymph volume and pressure can lead to the development of deafness and balance disorders. Our previous work in zebrafish embryos has shown that the inner ear contains a pressure relief valve called the endolymphatic sac (ES) which opens to release excess endolymph and closes right after. How are ES cells capable of sensing changes in endolymph pressure to know when to open and close? It has been shown that specialized physiological tissues in the eye sense changes in ocular pressure through the activation of the mechanosensitive and Ca2+ permeable Piezo1 channel. Our preliminary microscopy data of zebrafish embryos confirms the presence of Ca2+ signals and Piezo1 channels in ES cells. Thus, to characterize the role of Ca2+ signaling through Piezo1 activation in ES function we will be using a confocal live-imaging approach combined with genetic and drug perturbations in zebrafish embryos. This project consists of the development of zebrafish mutant and transgenic lines which will be used further for confocal live-imaging and potentially quantification of the resulting imaging data.
Qualifications: - Students who are looking for their first research experience should demonstrate capacity to learn laboratory techniques in their application (e.g. completion of a laboratory class, strong grades in STEM courses, an interest in data analysis)
- An ideal candidate is detail-oriented, highly motivated, curious, and passionate about scientific discovery, and has a clear vision for how a URAP experience will aid them in their career goals.
- Sophomores preferred, but freshmen and juniors with strong qualifications will be considered as well.
Day-to-day supervisor for this project: María Torres-Colón, Ph.D. candidate
Hours: 12 or more hours
Related website: http:///www.swinburnelab.org/
Biological & Health Sciences