Multi-photon Microscopy for Imaging Vascular Pathogenesis and Regeneration in Mouse Models
Rong Wang, Professor
UC San Francisco
Applications for Fall 2024 are closed for this project.
The vasculature supplies all cells in the body. A dysfunctional vasculature causes myriad diseases, including stroke, arterial occlusive diseases, hemorrhages, and vascular anomalies. Our long-term goal is to understand vessel Pathogenesis and Regeneration and to treat vascular diseases. Our strategy is to understand genes crucial for angiogenesis (new vessel formation) in the normal and diseased states. We employ cutting-edge mouse genetics to delete or express genes in a temporally controllable fashion in vascular cells. We combine these molecular approaches with mouse models of diseases and live 5D two-photon imaging (3D + blood flow over time) to uncover both the molecular mechanisms and hemodynamic signals in normal development and disease progression/regression.
Qualifications: Role:
Learn and assist in operating the custom-built two-photon microscope, utilizing MATLAB software to command the microscope, maintaining the microscope, including Laser, Chiller, filter, etc; optical maintenance, laser tracing/alignment, component switch and assembly, software hardware connections, guided trouble shooting, communicating with collaborators (including physicists, engineers, and biologists) and vendors, obtaining imaging data, data analysis, ppt preparation, literature search/summaries/citations. Opportunities to earn authorship in publications.
Qualifications:
1. A research career goal in BioE or applied physics fields
2. A major or background in Engineering or physics.
3. Highly responsible, motivated, dependable, accurate, meticulous and punctual.
4. Are interested in staying with the lab for at least one year or longer
5. Able to come to UCSF at least 2 days a week (hours to be negotiated)
6. Enthusiastic to work with custom-design in lab constructed two-photon microscope
7. MATLAB knowledge/ability
8. Willing to work with mice or collaborate with mouse scientists
9. Have taken upper division science courses
10. Strong hands-on, organizational, and communication skills
11. Fast learner who invites feedback for improvements
12. Prior Research experiences and strong writing/editing skill are a plus
13. Students who aim to pursue a Ph.D degree are encouraged to apply.
Day-to-day supervisor for this project: Professor Rong Wang and TBN
Hours: 12 or more hours
Off-Campus Research Site: UCSF
Related website: http://