Iain Clark

Closed (1) Ultra-high throughput sorting techniques to study rare cells

Closed. This professor is continuing with Spring 2021 apprentices on this project; no new apprentices needed for Fall 2021.

This project will develop a microfluidic device to generate water-in-oil-in-water double emulsion droplets. These picoliter compartments will be loaded with single cells, assayed, and sorted. The goal of this project is to isolate and study rare cells that control CNS inflammation in neurologic diseases like Multiple Sclerosis.

This work will entail learning soft-lithography, fabricating microfluidic devices, and developing cell-based assays.

Qualifications: A desire to learn microfabrication techniques.

Weekly Hours: 9-11 hrs

Closed (2) Genome-wide CRISPR screens to study host-virus interactions

Closed. This professor is continuing with Spring 2021 apprentices on this project; no new apprentices needed for Fall 2021.

This work will contain cell culture, molecular biology, and assay development. We plan to transduce reporter cell lines with genome-wide CRISPR libraries and screen these libraries for host factors that control viral activation.

Qualifications: A desire to learn molecular and cell culture techniques

Weekly Hours: 9-11 hrs

Closed (3) Bioinformatic analysis of cell-cell interactions

Closed. This professor is continuing with Spring 2021 apprentices on this project; no new apprentices needed for Fall 2021.

This project will develop the computational framework to analyze a novel spatial transcriptomic dataset. We recently developed a molecular method that categorizes the cellular connections and transcriptional profiles of glial cells in the mouse brain. The data from this technique can be represented as a highly connected network. The goal of this project is to discover ligand-receptor interactions that promote inflammation in the brain using network analysis.

Qualifications: Programming experience, preferably in R

Weekly Hours: to be negotiated

Off-Campus Research Site: Anywhere with internet access