George A. Brooks, Professor

Closed (1) Aging Research, Project 1: Cellular and Molecular Studies of Mitochondrial Fragmentation and loss of Metabolic Flexibility in Aging

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Projects 1 (Cell and Molecular Regulation of Metabolism) and 2 (Whole body Metabolic Regulation) are related. The student will be working on a large interdisciplinary research project investigating the effects of aging, gender and physical fitness on the control the balance of energy derived from different fuel sources within the body. Focus will be on the effects of lactate on metabolic flexibility. Specifically, in Project 1 the student will become facile in the care and handling of lab rodents as well as using with cellular and molecular tools and technologies to study metabolic regulation. In Project 2 in human subjects the student will become facile with taking vital signs, exercise stress testing, pulmonary function assessment, indirect calorimetry, and ECG recording and blood pressure determination. As well, in both projects the student will learn safe laboratory procedures such as decanting blood and managing it through various spectrophotometric, enzymatic and GC/MS analyses. The student will gain experience working with a research team including professor (principal investigator), staff research associates, post-doctoral fellows, graduate student researcher, attending physician, nurse and human subjects (Project 2). Importantly, the student will gain experience in the concepts and practices of ethical human subject research.

Students will need to take online instruction in EHS 101 on safe laboratory practices and other courses through EH&S and CITI.

To be determined, see above.

Day-to-day supervisor for this project: Justin Duong, MS, SRA, Staff Researcher

Qualifications: Campus courses on biology, physiology, exercise physiology, nutrition and metabolism, IB 132 and IB 123AL past or concurrent desired. Off campus certifications: First Aid, Paramedic, PA, other.

Weekly Hours: to be negotiated

Off-Campus Research Site: Two hours/Semester unit, minimum of 2 SUs (6 hr/wk) in Campus

Related website: https://ib.berkeley.edu/people/faculty/brooksg

Closed (2) Exercise Metabolism, Project 2: Stable Isotopes in the Study of Metabolism in Aging

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Projects 1 (Cell and Molecular Regulation of Metabolism) and 2 (Whole body Metabolic Regulation) are related. The student will be working on a large interdisciplinary research project investigating the effects of aging, gender and physical fitness on the control the balance of energy derived from different fuel sources within the body. Focus will be on the effects of lactate on metabolic flexibility. Specifically, in Project 1 the student will become facile in the care and handling of lab rodents as well as using with cellular and molecular tools and technologies to study metabolic regulation. In Project 2 in human subjects the student will become facile with taking vital signs, exercise stress testing, pulmonary function assessment, indirect calorimetry, and ECG recording and blood pressure determination. As well, in both projects the student will learn safe laboratory procedures such as decanting blood and managing it through various spectrophotometric, enzymatic and GC/MS analyses. The student will gain experience working with a research team including professor (principal investigator), staff research associates, post-doctoral fellows, graduate student researcher, attending physician, nurse and human subjects (Project 2). Importantly, the student will gain experience in the concepts and practices of ethical human subject research.
Students will need to take online instruction in EHS 101 on safe laboratory practices and other courses through EH&S and CITI.


To be determined, see above.

Day-to-day supervisor for this project: Michael A. Horning, Staff Researcher

Qualifications: Campus courses on biology, physiology, exercise physiology, nutrition and metabolism, IB 132 and IB 123AL past or concurrent desired. Off campus certifications: First Aid, Paramedic, PA, other.

Weekly Hours: to be negotiated

Off-Campus Research Site: Two hours/Semester unit, minimum of 2 SUs (6 hr/wk) on Campus.

Related website: https://ib.berkeley.edu/people/faculty/brooksg

Closed (3) Exercise Metabolism, Project 3: Regulation of Muscle Cell Membrane Lactate Transporter Protein Expression

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We are in the process of identifying the physiological signals responsible for lactate transporter expression. For this purpose when have developed a muscle cell culture system to study the influences of various putative factors in lactate transporter gene and protein expression. This project can cell culture, Western bottling (for protein detection), and RT-PCR for assessment of mRNA and pre-mRNA detection . We anticipate the time commitment for this job to be 6 hours/week (2 credits).

URAP students applying for this project should have taken, or be currently enrolled in, Integrative Biology 123A or a comparable upper level course that focuses on human metabolism. In addition, some familiarity with basic lab procedures (such as pipetting, making solutions, etc.) is also required. This is excellent training for a student interested in a career in biotechnology.

Students will need to take instruction in EHS 101 and safe laboratory practices and human laboratory animal care and handling.

Qualifications: IB 132 and IB 123A concurrent.

Weekly Hours: to be negotiated

Off-Campus Research Site: Two hours/Semester unit, minimum of 2 SUs (6 hr/wk).

Related website: https://ib.berkeley.edu/people/faculty/brooksg

Closed (4) Project 4: Metabolic Adaptions following Traumatic Brain Injury

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The applicant will work with GSI/GSR Austin Peck on the discovery of metabolic signaling following Traumatic Brain Injury (TBI) to rats and mice. Responsibilities will run the full gambit from assisting in animal handling (including care and feeding), to data collection and analysis. Injured and uninjured control animals will be subjected to behavioral tests as well as numerous tests on living and postmortem animals. The techniques will include aspects of MRI on live animals and confocal microscopy on histological sections. As well, metabolic signaling pathways will be tracked via gene array analysis and Western blotting and Mass spectrometry. Microscopy and polarography studies will be used to determine the effects TBI on brain and muscle mitochondrial morphology and function. , Ph.D. candidate

Qualifications: IB 132 and IB 123A concurrent. Students will need to take instruction in safe laboratory practices and humane laboratory animal care and handling.

Weekly Hours: to be negotiated

Related website: https://ib.berkeley.edu/people/faculty/brooksg
Related website: http://www.chori.org/Principal_Investigators/Kuypers_Frans_A/kuypers_overview.html