Guo Huang, Professor

Closed (1) Molecular control of organ regeneration in zebrafish and mice (students from under-representative backgrounds are particularly encouraged to apply)

Applications for fall 2021 are now closed for this project.

As shown in our recent publication (Hirose*, Payumo*, et al Science 2019), we aim to understand the divergent regenerative potential in ontogeny and phylogeny. For example, heart regeneration is remarkably robust in adult zebrafish and newborn mice while very limited in adult mammals. We use the heart as a model system and an entry point to study organ regeneration and repair in adult zebrafish, neonatal and adult mice, with an emphasis on the pathways that regulate resident stem cell activation and mature cell dedifferentiation/proliferation, and with innovative and integrated approaches in engineering, single cell analysis, advanced imaging microscopy and genome manipulation technology.

Specific questions include:

1. How does the heart regenerate in newborn mice and adult zebrafish?

2. What triggers the loss of cardiac regenerative potential in the mammalian postnatal life?

3. Can we reactivate regenerative potentials in adult mammalian hearts, and if so, how?

4. Are there species in higher vertebrates with extraordinary yet previously unknown tissue regenerative capacity?

5. Are there any human conditions or diseases that may result in preservation of cardiac regenerative potential? If so, how?

Our research will shed lights on the fundamental principle governing organ regeneration in development and evolution, and may suggest novel approaches of regenerative medicine.

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Publications from previous URAP students [name]:

1. Hirose, K.*, Payumo, A. Y.*, Cutie, S., [Hoang, A.], et al., and Huang, G. N. (*, equal contribution) (2019 Apr) Evidence for hormonal control of heart regenerative capacity during endothermy acquisition. Science

2. Cutie, S., [Hoang, A. T.], Payumo, A. Y., and Huang, G. N. (2017 Dec) Unconventional functions of muscles in planarian regeneration. Developmental Cell

3. Hirose, K., [Chang, S.], et al., and Huang, G. N. (Submitted) Loss of a novel striated muscle-enriched mitochondrial protein Coq10a enhances postnatal cardiac hypertrophic growth.

4. [Amram, A.], Cutie, S., and Huang, G. N. (2021 Jan) Hormonal control of cardiac regenerative potential. Endocrine Connections

5. [Khyeam, S.], [Lee, S.], and Huang, G. N. (2021 May) Genetic, epigenetic, and postā€transcriptional basis of divergent tissue regenerative capacities among vertebrates. Advanced Genetics, e10042

6. Payumo, A. Y.*, Chen, X.*, Hirose, K., Chen, X., Hoang, A., [Khyeam, S.], Yu, H., Wang, J., Chen, Q., [Powers, N.], Chen, L., Bigley, R. B., Lovas, J., Hu, G., & Huang, G. N. (*, equal contribution) (2021 Sep) Adrenergic-thyroid hormone interactions drive postnatal thermogenesis and loss of mammalian heart regenerative capacity. Circulation
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Candidates will work with postdoctoral fellows and staff researchers in the lab. Students will also have the opportunity to engage directly with the professor.

Potential techniques and experience acquired during the training include:
- Advanced imaging microscopy on live or fixed tissues and animals;
- Molecular cloning and genome editing using the CRISPR technology;
- Mammalian and zebrafish cell culture;
- Mice and zebrafish husbandry, genotyping, and injury/regeneration models.

Day-to-day supervisor for this project: Shea Khyeam, Staff Researcher

Qualifications: This position is open to any freshmen, sophomores, juniors and seniors who are highly motivated and very organized. Please include in the application (1) your career plan, (2) daily schedule of committed hours to perform research in the lab (please exclude the commute time), (3) prior research experience if any, (4) (optional) experience of photography, painting or related experience that demonstrates strong visual artistic senses if any, (5) (optional) a link to your LinkedIn profile if available, (6) (optional) a one-sentence description if you are from diverse backgrounds in but not limited to ethnicity, sexual orientation, and geography (including international students with F1 visa).

Weekly Hours: 12 or more hours

Off-Campus Research Site: 555 Mission Bay Blvd S, San Francisco, CA 94158

Related website: http://www.cvri.ucsf.edu/~huang/lab/Research.html