Exercise Metabolism, Project 3: Regulation of Muscle Cell Membrane Lactate Transporter Protein Expression
George A. Brooks, Professor
Integrative Biology
Closed. This professor is continuing with Fall 2023 apprentices on this project; no new apprentices needed for Spring 2024.
We are in the process of identifying the physiological signals responsible for lactate transporter expression. For this purpose when have developed a muscle cell culture system to study the influences of various putative factors in lactate transporter gene and protein expression. This project can cell culture, Western bottling (for protein detection), and RT-PCR for assessment of mRNA and pre-mRNA detection . We anticipate the time commitment for this job to be 6 hours/week (2 credits).
URAP students applying for this project should have taken, or be currently enrolled in, Integrative Biology 123A or a comparable upper level course that focuses on human metabolism. In addition, some familiarity with basic lab procedures (such as pipetting, making solutions, etc.) is also required. This is excellent training for a student interested in a career in biotechnology.
Students will need to take instruction in EHS 101 and safe laboratory practices and human laboratory animal care and handling.
Qualifications: IB 132 and IB 123A concurrent.
Hours: to be negotiated
Off-Campus Research Site: Two hours/Semester unit, minimum of 2 SUs (6 hr/wk).
Related website: https://ib.berkeley.edu/people/faculty/brooksg
Biological & Health Sciences