Structural mechanism of human kinetochore assembly on microtubules
Eva Nogales, Professor
Molecular and Cell Biology
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Accurate segregation of chromosomes during cell division is essential for maintaining proper cellular function and organismal health. Errors in chromosome segregation can result in apoptosis or an abnormal chromosome number, leading to conditions such as Down syndrome and Turner syndrome, exposing detrimental recessive mutations, and being associated with cancer. The kinetochore is a critical multiprotein complex that facilitates the attachment of chromosomes to microtubules (MTs) and harnesses the force generated by MT depolymerization to ensure the correct segregation of sister chromatids. However, the molecular mechanisms underlying this complex machinery remain not fully understood. Our research is particularly focused on elucidating how the outer kinetochore protein complexes coordinate with each other on MTs. To achieve this, we are employing cryo-electron microscopy (Cryo-EM) to study the structural dynamics and interplay of outer kinetochore complexes and their coordination on MTs, aiming to provide insights into the mechanistic basis of chromosome segregation by MTs.
Role: The candidate would 1) Participate in the cloning and purification of recombinant proteins. 2) Learn and participate in sample preparation for negative-staining and Cryo-EM. 3) Gain knowledge and experience in cryo-EM and data processing.
Qualifications: The applicant ideally would have foundational understanding of experimental molecular biology, biochemistry, or structural biology, along with a strong motivation to learn about protein purification and Cryo-EM. Additionally, good analytical and organizational skills, critical thinking, and a desire to participate in cutting-edge research as a team are required!
Day-to-day supervisor for this project: Ju Zhou, Post-Doc
Hours: 12 or more hours
Related website: http://cryoem.berkeley.edu
Biological & Health Sciences