Determining what factors influence the microbiome of wild populations of birds and mammals
Rauri Bowie, Professor
Integrative Biology, Museum of Vertebrate Zoology
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My lab is involved with a large collaborative project to study disease transmission within and among species of wild birds. As part of this project, swabs of a variety of bird species are being collected to study their microbial communities (i.e. their microbiome). Lab studies suggest that microbial diversity can be linked with health, so we will be testing whether the microbiome in wild birds is affected by disease status and if microbial diversity is correlated with indicators of health (e.g. body size, plumage variation). In addition, we will be using data on the microbiome to model the spread of diseases within and among bird species. We are also interested in testing what general factors affect the microbiomes of birds and small mammals.
Role: The main role for the student would be to assist with the extraction of microbial DNA from field collected samples from birds. The student would need to learn basic molecular genetics techniques, such as how to extract, quantify, and visualize DNA. They would also need to adopt sterile techniques for lab work, because contamination of the samples from outside sources is a large concern. Students who show a strong interest in the project will be invited to become involved in the analyses of the microbial data.
Qualifications: This project would be good for students with a strong interest in learning laboratory skills and techniques for assessing microbial diversity. Good organizational skills and high levels of attention to detail in the lab is a must. Previous laboratory experience is not required, but students with prior experience are encouraged to apply, as they will be able to make more rapid progress in the lab. The DNA extraction protocol requires being in the lab for 6-8 hours. Thus, students need to have sufficient time open in their schedules to permit an extended stretch of lab work. Students will primarily be mentored by Ammon Corl, a postdoctoral researcher in the lab.
Day-to-day supervisor for this project: Jackie Childers, Post-Doc
Hours: 9-11 hrs
Biological & Health Sciences