Functional genetics of sexual isolation in Drosophila
Doris Bachtrog, Professor
Integrative Biology
Applications for Fall 2026 are closed for this project.
How do new species form? One of the major challenges in evolutionary biology is identifying the genetic changes that cause populations to stop mating with one another. We study this question in Drosophila athabasca, a group of closely related fruit fly populations that are at a very early stage of speciation. These populations can still produce fertile offspring, but males produce strikingly different courtship songs and females preferentially mate with males from their own population.
Our previous genetic and genomic work identified candidate genes that may control these behavioral differences, including the gene nonA, but until recently we did not have the tools to test these candidates directly in D. athabasca. Our lab has now developed methods for genetic manipulation of several non-model Drosophila species using CRISPR, transgenesis, and a robotic embryo microinjection system.
The goal of this project is to establish these techniques in D. athabasca and directly test genes involved in courtship behavior and reproductive isolation. Ultimately, we hope to connect specific genetic changes to differences in male courtship song, female mating preference, and the formation of new species.
Role: The undergraduate apprentice will work closely with members of the lab to help establish and maintain D. athabasca as a new experimental genetic system. Depending on experience and progress, activities may include:
• Maintaining Drosophila stocks and setting up genetic crosses
• Collecting virgin males and females for genetic and behavioral experiments
• Preparing embryos and flies for transgenic/CRISPR experiments
• Screening offspring from genetic manipulations
• DNA extraction, PCR, and genotyping of candidate transgenic or mutant flies
• Assisting with courtship and mating assays
• Recording and analyzing behavioral data, including male courtship song
• Organizing and analyzing experimental data
Students will learn basic Drosophila genetics, experimental design, molecular-genetic techniques, and approaches for studying animal behavior and evolution. More advanced students may have opportunities to participate in CRISPR experimental design, analysis of sequencing data, and independent experiments.
Qualifications: Required: Reliability, attention to detail, and willingness to work carefully with live flies. Students must be able to commit to a regular weekly schedule because many experiments depend on the timing of fly development.
Desirable but not essential: Previous coursework in genetics, evolution, molecular biology, or animal behavior. Prior laboratory or Drosophila experience is helpful but not required; appropriate techniques will be taught.
Hours: 12 or more hours
Biological & Health Sciences