Exploring viral dynamics between Honeybees, almond flowers, and pollen
Mike Boots, Professor
Integrative Biology
Applications for Spring 2025 are closed for this project.
Our research group in the Department of Integrative Biology studies the dynamics, ecology, and evolution of infectious diseases through various systems.
Almonds are majorly grown in California and require mass imports of honeybees for their pollination. However, honeybees used for mass agriculture also suffer from constant exposure to pathogens. RNA viruses such as deformed wing virus (DWV) pose a major risk to pollinators, such as honeybees, and are associated with colony collapse. Pollen has been shown to play a role in transmitting honeybee-associated viruses when vectored between healthy and infected individuals sharing floral resources. Likewise, honeybees may also play a role in the spread of plant disease through the act of pollination. The virome of pollen and its role in vectoring diseases is still not fully understood. Furthermore, understanding the mechanisms by which RNA viruses transmit through pollinator interactions is an important part of mitigating risks and harm to our food systems. As a research assistant, you will work alongside a graduate student to help process pollen/flower samples for the assessment of viruses and build a pollen library. There is a potential to expand this project and work with wildflowers/pollen collections from the Sierra Nevada.
Role: This student will gain experience in a wet lab setting. The student will assist in a variety of tasks in the lab, primarily focused on RT-PCR techniques, including, extraction, cDNA synthesis, etc.. The student will also aid in optimizing pollen-washing experiments and help construct a pollen library using microscopy techniques. The student will be expected to read scientific literature with relevant information pertaining to the project.
Qualifications: The ideal candidate is interested in practicing/learning molecular and microscopic techniques and is excited to learn more about pollinator disease dynamics. No prior experience is required, but a preference for individuals with a focus in insect/plant/pollinator science. Desired qualifications include good time management, strong communication skills, knowledge of general biology, and enthusiasm for pollinators.
Day-to-day supervisor for this project: Sara Herrejon Chavez
Hours: 3-5 hrs
Related website: https://bootslab.org/
Biological & Health Sciences