Understanding interneuron hypofunction in mouse models of autism
Dan Feldman, Professor
Molecular and Cell Biology
Closed. This professor is continuing with Fall 2023 apprentices on this project; no new apprentices needed for Spring 2024.
This neuroscience project will use immunohistochemistry to test for abnormalities in inhibitory interneurons in cerebral cortex in mouse models of autism. Interneuron hypofunction is common in mouse models of autism, but how this impairment in inhibition arises is unclear. We focus on two interneuron types, parvalbumin-positive interneurons (PV cells) which are involved in feedforward circuits, and somatostatin interneurons (SST cells) which play critical roles in local feedback inhibition. This project will test whether PV and/or SST cell number is reduced in several transgenic mouse models of autism, or whether expression of parvalbumin and/or somatostatin protein markers is altered in these cells. Either of these phenotypes, if present, could lead to aberrant inhibition in sensory cortex.
Role: Over the course of this project, students will gain proficiency in brain slice preparation, immunohistochemical techniques, confocal microscopy, and image analysis. These skills will be applied to understanding the cellular underpinnings of autism spectrum disorders.
Additionally, students will learn to read primary research papers and review articles, and to synthesize information from these sources to develop and improve methods for imaging/analysis in the lab. Students will periodically present their progress on the project and will create documentation of methods for future users.
Contributions to the advancement of the project may lead to inclusion as an author on resulting conference abstracts and publications.
Qualifications: An interest in understanding neuron function, autism, and imaging methods such as confocal microscopy. Experience with wet lab techniques especially brain slicing, immunostaining or imaging techniques such as fluorescence microscopy are a bonus. A minimum commitment of 12 hours/week is expected. Days of the week when the student can work are flexible and can include the weekend, depending on the preference of the student. Working with brain tissue and using the microscope involves extensive initial training which will take up a considerable portion of the current semester, therefore this project will be most suitable for students interested in continuing this work in future semesters. Students should be proactive at problem-solving, and maintain clear communication with the supervisor and team about the project.
Day-to-day supervisor for this project: Hannah Monday, Bree Bohannon, Post-Doc
Hours: 12 or more hours
Biological & Health Sciences